New assay systems for lipid hydroperoxides. Quantitative analysis of lipid hydroperoxides by an HPLC/chemiluminescence method.
نویسندگان
چکیده
منابع مشابه
Lipid hydroperoxides in plants.
Hydroperoxides are the primary oxygenated products of polyunsaturated fatty acids and were determined spectrophotometrically based on their reaction with an excess of Fe2+ at low pH in the presence of the dye Xylenol Orange. Triphenylphosphine-mediated hydroxide formation was used to authenticate the signal generated by the hydroperoxides. The method readily detected lipid peroxidation in a ran...
متن کاملPreparation of pure lipid hydroperoxides.
Increasing evidence of lipid peroxidation in food deterioration and pathophysiology of diseases have revealed the need for a pure lipid hydroperoxide (LOOH) reference as an authentic standard for quantification and as a compound for biological studies in this field. Generally, LOOH is prepared from photo- or enzymatically oxidized lipids; however, separating LOOH from other oxidation products a...
متن کاملDetermination of Lipid Hydroperoxides in Marine Diatoms by the FOX2 Assay
Ecologically-relevant marine diatoms produce a plethora of bioactive oxylipins deriving from fatty acid oxidation, including aldehydes, hydroxy-fatty acids, epoxy-hydroxy-fatty acids, and oxo-acids. These secondary metabolites have been related to the negative effect of diatoms on copepod reproduction, causing low hatching success and teratogenesis in the offspring during periods of intense dia...
متن کاملA Highly Sensitive Method for Measurement of Lipid Hydroperoxides by Iodimetry and Amperometric Endpoint.
T h i s report describes a procedure that permits accurate and specific measurement of lipid peroxides by iodimetry in the small samples available in many biological studies. Martin’s review (1) of methods for determination of organic peroxides indicates that most methods are based on reactions with various reducing substances. The most serious drawback of methods other than iodimetry can be as...
متن کاملEvidence for a concerted reaction between lipid hydroperoxides and polypeptides.
The events accompanying oxidative modification of low density lipoprotein (LDL) are multiple and complex, and the precise mechanisms remain to be determined. In the present studies, we examined a simple system in which we first prepared large amounts of lipid hydroperoxides (from linoleic acid or from phospholipids containing linoleic acid) by using soybean lipoxygenase (linoleate:oxygen oxidor...
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ژورنال
عنوان ژورنال: Journal of the agricultural chemical society of Japan
سال: 1988
ISSN: 0002-1407,1883-6844
DOI: 10.1271/nogeikagaku1924.62.1495